Laccases are enzymes belonging to the group of oxidases. Aspergillus terreus KJ829487 obtained from cassava peels have the ability to produce high concentration cellulases and xylanases which effectively hydrolysed the lignocelluloses' biomass to fermenta-ble sugars. Saccharifica-tion of the peels yielded 584 mg/L glucose, 78 mg/L xylose and 66 mg/L rhamnose. It produces cellulases and xylanases optimally at 40˚C, pH 6 and 8, utilising carboxymethylcellulose (CMC) or xylose and yeast extracts as its carbon and nitrogen sources respectively. Aspergillus terreus KJ829487 was the predominant fungus. Optimisation of growth conditions for enzymes production was monitored using the DNSA method, also saccharification of cassava peel were carried out using the enzymes obtained from the isolate. Isolate with highest frequency of occurrence and enzyme production was identified using phenotypic and molecular method. Fungi were isolated, identified and screened for cellulase and xylanase production. Decaying parts of cassava peels were obtained and brought to the laboratory for further work. The aim of this study is to screen fila-mentous fungi from decaying cassava peels that are good producers of xylanases and cellulases. This lignocellulolytic biomass can be converted using microbial enzymes to fermentable sugars which have wide range of biotechnological relevance in many fermentation processes. Its peels are made up of cellulose, hemicellulose and lignin. Keywords: Wood decaying brown rot fungi wastewater decolorization malt extract agarĬassava (Manihot esculenta, Crantz) is one of the most important food plants in West Africa. Isolated BRF species can be used to explore their potential of biodegradation of textile dyes. Two of these are common brown rot fungi belong to basidomycota and three are un-common brown rot fungi belong to ascomycota.Ĭonclusion: Rotten wood is a very good source of brown rot fungi. Results: More than 20 species were collected, out of these 05 were cultured on Malt Extract Agar media and identified on morphological basis. Of Plant Pathology, PMAS-Arid Agriculture University Rawalpindi Pakistan for morphological identification by mycologist.
Collected fungi species were cultured on Malt Extract Agar media at pH 5.5 and pure culture plates were submitted in Mycology Laboratory, Department Methodology: The current study was conducted to collect Brown Rot Fungi (BRF) species fromrotten wood and their morphological identification. More and more new species so that these can be used for beneficial processes. Many studies have beenĬonducted to explore their potential for successful industrialization. Productions, dyes biodegradation and wastewater decolorization. This study contributes to integrated TLB management options, and to make a valid recommendation for TLB management strategy, the study should be repeated over years and locations where TLB of maize is of major economic importanceĪims: Wood decaying fungi are very good candidate for industrial processes like enzymes The highest marginal benefit (ETB 48,801.28 ha-1) and marginal rate of return (ETB 6.33) were obtained from propiconazole-treated varieties BH-543 and BH-546, respectively. Percent severity index, AUDPC, incidence and disease progress rates were negatively correlated with yield components regardless of grain yield loss. Turcicum leaf blight resulted in grain yield losses of up to 40.7% on the unsprayed plots of the susceptible variety BH-543. The highest (11383 kg ha-1) grain yield was obtained from propiconazole-treated hybrid maize variety BH-546. Integration effects of varieties with fungicides significantly affected the grain yield and thousand kernel weight (TKW) of maize varieties. Disease severity was scored using 1 to 5 scale on 12 randomly-tagged plants in the central rows. Unsprayed plots were left as control or check for each variety.
turcicum was inoculated at third-fifth leaves. A pinch of ground maize leaf infected by E. The experiment was arranged in 3 × 6 factorial combinations in split plot design with three replications. The field experiment was conducted at Bako Agricultural Research Center in 2014 main cropping season using six maize varieties (BH-540, BH-543, BH-546, BH-660, BH-661 and AMHQ-760) integrated with foliar sprays of the systemic fungicide propiconazole (Tilt) at the rate of 350 ml ha-1and the contact fungicide mancozeb (Dithane M-45) at 2.6 kg ha-1. The objectives of this study were to determine the effect of maize varieties integrated with fungicides on epidemics of turcicum leaf blight to determine the effect of turcicum leaf blight severity on yield and yield components of maize and to assess the cost and benefit of using fungicides. Turcicum leaf blight (TLB) (Exserohilum turcicum) is a major disease affecting maize production in western Ethiopia.
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